Use RNA-seq for obtaining inventories of genes expressed in three wireworm pests. ID of potential target genes specific to each of the species and design dsRNAs for knockdown. Optimize CPP-mediated gene expression knock-down and suppression of feeding to ID best dsRNAs. Demonstrate RNAi protection of wheat seeds and seedlings in controlled environment.
Lead Researcher (PI): John Laurie
Institution: Agriculture & Agri-Food Canada
Total WGRF Funding: $138,065
Co-Funders: Alberta Wheat Commission, Results Driven Agriculture Research, Saskatchewan Wheat Development Commission
Start Date: 2021
Project Length: 4 Years